Discovering therapeutic antibodies designed by nature

PNAS publication

PNAS publication

December 11, 2014

Published in PNAS: 2014 Nov 25; 111(47):16820-5.

A bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity.

Bispecific antibodies greatly enhance the therapeutic potential of antibodies, because they can bind simultaneously two or multiple antibody targets.
Until now, many different formats of bispecific antibodies have been developed. Most of these are based on genetic modifications of the antibody backbone in order to facilitate incorporation of multiple variable domains in a single molecule.
In this publication, AIMM scientists, in close collaboration with their colleagues at Whitehead Institute (MIT, Boston) and the Amsterdam Medical Center, describe a novel, scalable bispecific antibody format.
This bispecific antibody is generated through the C-to-C fusion of two antibodies. This fusion is an elegant two-step process utilizing sortase transpeptidation and click-chemistry.
This proprietary process (AIMLink) is fully validated by fusing two potent, subtype specific, antiinfluenza antibodies, AT10-002 and AT10-005, resulting in a potent bispecific pan influenza antibody.