- are highly stable
- proliferate rapidly
- express surface immunoglobulin
- secrete useful quantities of antibody and
- can be frozen and thawed without loss of antibody producing capacity
These characteristics make it possible to select relevant monoclonal antibodies among a plethora of antibodies produced by the immortalized cells. Selection can be conducted by binding a labeled antigen to the surface immunoglobulin or by measuring the specific antibody functional activity (i.e., binding, neutralization).