Publications

Publications

Blood. 2017 Oct 23
AML-specific cytotoxic antibodies in patients with durable graft versus leukemia responses.
Gillissen MA, Kedde M, de Jong G, Moiset G, Yasuda E, Levie SE, Bakker AQ, Claassen YB, Wagner K, Böhne M, Hensbergen PJ, Speijer D, van Helden PM, Beaumont T, Spits H, Hazenberg MD.

J Immunol Methods. 2017 Aug 27. Epub ahead of print
Multiplex flow cytometry-based assay to study the breadth of antibody responses against E1E2 glycoproteins of hepatitis C virus.
Merat SJ, van de Berg D, Bru C, Yasuda E, Breij E, Koostra N, Prins M, Molenkamp R, Bakker AQ, de Jong MD, Spits H, Schinkel J, Beaumont T.

Blood advances. 2017 1:1551-1564
A patient derived antibody recognizes a unique CD43 epitope expressed on all AML and has anti leukemia activity in mice.
Gillissen MA, de Jong G, Kedde M, Yasuda E, Levie SE, Moiset G, Hensbergen PJ, Bakker AQ, Wagner K, Villaudy J, van Helden PM, Spits H, Hazenberg MD.

Bioconjug Chem. 2017 Apr 19;28(4):1189-1193.
Inducible, Site-Specific Protein Labeling by Tyrosine Oxidation-Strain-Promoted (4 + 2) Cycloaddition.
Bruins JJ, Westphal AH, Albada B, Wagner K, Bartels L, Spits H, van Berkel WJH, van Delft FL.

J Virol. 2017 Apr 28;91(10).
Broadly reactive anti-RSV G antibodies from exposed individuals effectively inhibit infection of primary airway epithelial cells
Cortjens B, Yasuda E, Yu X, Wagner K, Claassen YB, Bakker AQ, van Woensel JB, Beaumont T.

PLoS One. 2016 Oct 24; 11: e0165047.
Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated 25 Years after Spontaneous Clearance
Merat SJ, Molenkamp R, Wagner K, Koekkoek SM, van de Berg D, Yasuda E, Böhne M, Claassen YB, Grady BP, Prins M, Bakker AQ, de Jong MD, Spits H, Schinkel J, Beaumont T.

PLoS One. 2016 Oct 5;11(10).
The Production of a Stable Infliximab Powder: The Evaluation of Spray and Freeze-Drying for Production
Kanojia G, Have RT, Bakker A, Wagner K, Frijlink HW, Kersten GF, Amorij JP.

Nat Commun. 2016 Jul 20;7:11387.
Multiple capsid-stabilizing interactions revealed in a high-resolution structure of an emerging picornavirus causing neonatal sepsis
Shakeel S, Westerhuis BM, Domanska A, Koning RI, Matadeen R, Koster AJ, Bakker AQ, Beaumont T, Wolthers KC, Butcher SJ.

Nature. 2016 Aug 4;536(7614):91-5.
Neoantigen landscape dynamics during human melanoma-T cell interactions
Verdegaal EM, de Miranda NF, Visser M, Harryvan T, van Buuren MM, Andersen RS, Hadrup SR, van der Minne CE, Schotte R, Spits H, Haanen JB, Kapiteijn EH, Schumacher TN, van der Burg SH.

Bone Marrow Transplant. 2016 Sep;51(9):1245-8.
AML relapse after rituximab treatment for GvHD: crucial role for B cells in GvL responses
Gillissen MA, de Jong G, Levie SE, Yasuda E, Bakker AQ, Evers LM, Pals ST, Huisman C, van Helden PM, Spits H, Hazenberg MD.

J Immunol Methods. 2016 Jul; 434 16-23.
The modified FACS calcein AM retention assay: a high throughput flow cytometer based method to measure cytotoxicity
Gillissen MA, Yasuda E, De Jong G, Levie SE, Go D, Spits H, van Helden PM, Hazenberg MD

Immunol Rev. 2016 March; 270(1) 65-77.
Stable long term cultures of self-renewing B cells and their applications
Kwakkenbos MJ, van Helden PM, Beaumont T, Spits H

J Virol. 2015 Sep; 89(18) 9571-80.
Structural Basis of Human Parechovirus Neutralization by Human Monoclonal Antibodies
Shakeel S, Westerhuis BM, Ora A, Koen G, Bakker AQ, Claassen Y, Wagner K, Beaumont T, Wolthers KC, Butcher SJ

J Virol. 2015 Aug; 89(15) 7457-64.
Human Memory B Cells Producing Potent Cross-Neutralizing Antibodies against Human Parechovirus: Implications for Prevalence, Treatment, and Diagnosis
Westerhuis BM, Benschop KS, Koen G, Claassen YB, Wagner K, Bakker AQ, Wolthers KC, Beaumont T

J Haematologica. 2015 Aug; 100(8).
Resistance to ABT-199 induced by microenvironmental signals in chronic lymphocytic leukemia can be counteracted by CD20 antibodies or kinase inhibitors
Thijssen R, Slinger E, Weller K, Geest CR, Beaumont T, van Oers MH, Kater AP, Eldering E

PLoS Pathog. 2015 Jul; 11(7).
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein
Gilman MS, Moin SM, Mas V, Chen M, Patel NK, Kramer K, Zhu Q, Kabeche SC, Kumar A, Palomo C, Beaumont T, Baxa U, Ulbrandt ND, Melero JA, Graham BS, McLellan JS

Nat Med. 2015 Jan; 21(1): 81-5.
High-throughput epitope discovery reveals frequent recognition of neo-antigens by CD4+ T cells in human melanoma
Linnemann C, van Buuren MM, Bies L, Verdegaal EM, Schotte R, Calis JJ, Behjati S, Velds A, Hilkmann H, Atmioui DE, Visser M, Stratton MR, Haanen JB, Spits H, van der Burg SH, Schumacher TN

Proc Natl Acad Sci U S A. 2014 Nov 25; 111(47): 16820-5
Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity
Wagner K, Kwakkenbos MJ, Claassen YB, Maijoor K, Böhne M, van der Sluijs KF, Witte MD, van Zoelen DJ, Cornelissen LA, Beaumont T, Bakker AQ, Ploegh HL, Spits H

Proc Natl Acad Sci U S A. 2014 Jan 7; 111(1): 445-50
A common solution to group 2 influenza virus neutralization
Friesen RH, Lee PS, Stoop EJ, Hoffman RM, Ekiert DC, Bhabha G, Yu W, Juraszek J, Koudstaal W, Jongeneelen M, Korse HJ, Ophorst C, Brinkman-van der Linden EC, Throsby M, Kwakkenbos MJ, Bakker AQ, Beaumont T, Spits H, Kwaks T, Vogels R, Ward AB, Goudsmit J, Wilson IA

Methods. 2014 Jan 1; 65(1): 38-43
Genetic manipulation of B cells for the isolation of rare therapeutic antibodies from the human repertoire
Kwakkenbos MJ, Bakker AQ, van Helden PM, Wagner K, Yasuda E, Spits H, Beaumont T

Science. 2013 Nov 1; 342(6158): 592-8
Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus
McLellan JS, Chen M, Joyce MG, Sastry M, Stewart-Jones GB, Yang Y, Zhang B, Chen L, Srivatsan S, Zheng A, Zhou T, Graepel KW, Kumar A, Moin S, Boyington JC, Chuang GY, Soto C, Baxa U, Bakker AQ, Spits H, Beaumont T, Zheng Z, Xia N, Ko SY, Todd JP, Rao S, Graham BS, Kwong PD

PLoS Pathog. 2013 9(10): e1003653
Novel Staphylococcal Glycosyltransferases SdgA and SdgB Mediate Immunogenicity and Protection of Virulence-Associated Cell Wall Proteins
Wouter L. W. Hazenbos, Kimberly K. Kajihara, Richard Vandlen, J. Hiroshi Morisaki, Sophie M. Lehar, Mark J. Kwakkenbos, Tim Beaumont, Arjen Q. Bakker, Qui Phung, Lee R. Swem, Satish Ramakrishnan, Janice Kim, Min Xu, Ishita M. Shah, Binh An Diep, Tao Sai, Andrew Sebrell, Yana Khalfin, Angela Oh, Chris Koth, S. Jack Lin, Byoung-Chul Lee, Magnus Strandh, Klaus Koefoed, Peter S. Andersen, Hergen Spits, Eric J. Brown, Man-Wah Tan, Sanjeev Mariathasan

Science. 2013 May 31; 340(6136): 1113-7
Structure of RSV fusion glycoprotein trimer bound to a prefusion-specific neutralizing antibody
McLellan JS, Chen M, Leung S, Graepel KW, Du X, Yang Y, Zhou T, Baxa U, Yasuda E, Beaumont T, Kumar A, Modjarrad K, Zheng Z, Zhao M, Xia N, Kwong PD, Graham BS

Proc Natl Acad Sci USA. 2011 Aug 9; 108(32): 13224-9. Epub 2011 Jul 25
Functional CD47/signal regulatory protein alpha (SIRP{alpha}) interaction is required for optimal human T- and natural killer- (NK) cell homeostasis in vivo
Legrand N, Huntington ND, Nagasawa M, Bakker AQ, Schotte R, Strick-Marchand H, de Geus SJ, Pouw SM, Böhne M, Voordouw A, Weijer K, Di Santo JP, Spits H

Science. 2011 Aug 12; 333 (6044): 843–850
A highly conserved neutralizing epitope on group 2 influenza A viruses
Ekiert DC, Friesen RHE, Bhabha G, Kwaks T, Jongeneelen M, Yu W, Ophorst C, Cox F, Korse HJWM, Brandenburg B, Vogels R, Brakenhoff JPJ, Kompier R, Koldijk MH, Cornelissen LAHM, Poon LLM, Peiris M, Koudstaal W, Wilson IA, and Goudsmit J

PLoS ONE. 2011 6(4); e17189
Antigen-Specific Monoclonal Antibodies Isolated from B Cells Expressing Constitutively Active STAT5
Scheeren FA, van Geelen CMM, Yasuda E, Spits H, and Beaumont T.

PLoS ONE. 2010: 5(10): e13137
Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated “Human Immune System” Mice
Becker PD, Legrand N, van Geelen CMM, Noerder M, Huntington ND, Lim A, Yasuda E, Diehl SA, Scheeren FA, Ott M, Weijer K, Wedemeyer H, Di Santo JP, Beaumont T, Guzman CA, Spits H

Nat Med. 2010 Jan; 16(1): 123-8.
Generation of stable monoclonal antibody–producing B cell receptor–positive human memory B cells by genetic programming
Kwakkenbos MJ, Dieh SA, Yasuda E, Bakker AQ, van Geelen CMM, Lukens MV, van Bleek GM, Widjojoatmodjo MN, Bogers WMJM, Mei H, Radbruch A,  Scheeren FA, Spits H, Beaumont T

J Clin Invest. 2010 Jan 4; 120(1): 214-22
CD20 deficiency in humans results in impaired T cell–independent antibody responses
Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IAM, Dolman KM, Beaumont T, Tedder TF, van Noesel CJM, Eldering E, van Lier RAW

J Immunol. 2008 Apr 1; 180(7): 4805-15
STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation
Diehl SA, Schmidlin H, Nagasawa M, van Haren SD, Kwakkenbos MJ, Yasuda E, Beaumont T, Scheeren FA, Spits H

Nat Immunol. 2005 Mar;6(3):303-13. Epub 2005 Feb 13
STAT5 regulates the self-renewal capacity and differentiation of human memory B cells and controls Bcl-6 expression
Scheeren FA, Naspetti M, Diehl S, Schotte R, Nagasawa M, Wijnands E, Gimeno R, Vyth-Dreese FA, Blom B, Spits H